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Petroleum Science >2011, Issue 3: 353-356 DOI: https://doi.org/10.1007/s12182-011-0152-y

Functional characterization of a biosurfactant-producing thermo-tolerant bacteria isolated from an oil reservoir Open Access

文章信息

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作者：Wang Jing,Ji Guang,Tian Jing,Zhang Hongdan,Dong Hanping and Yu Li

作者单位：

College of Chemical Engineering, China University of Petroleum, Beijing 102249, China;College of Chemical Engineering, China University of Petroleum, Beijing 102249, China;College of Chemical Engineering, China University of Petroleum, Beijing 102249, China;College of Chemical Engineering, China University of Petroleum, Beijing 102249, China;College of Chemical Engineering, China University of Petroleum, Beijing 102249, China;Langfang Branch, Research Institute of Petroleum Exploitation and Development, China National Petroleum Corporation, Hebei 065007, China

投稿时间：2010-12-15

引用方式：Wang, J., Ji, G., Tian, J. et al. Pet. Sci. (2011) 8: 353. https://doi.org/10.1007/s12182-011-0152-y

文章摘要

A Bacillus subtilis strain JA-1 isolated from an oil reservoir was studied. This strain is capable of growth and producing biosurfactant at a temperature of 60 oC. In nutrient medium it produced biosurfactant which reduced the surface tension from 68.2 mN/m to 28.3 mN/m, with the critical micelle concentration (CMC) of 48 mg/L. The measured surface tension indicated that the biosurfactant possessed stable surface activity at high temperature and a specific range of pH and salt concentrations. The results of thin layer chromatography (TLC) together with FT-IR showed that the metabolic product of strain JA-1 is a lipopeptide biosurfactant. The ability to growth at high temperature and to produce biosurfactant makes strain JA-1 promising for enhanced oil recovery.

关键词

Biosurfactant, bacillus subtilis, lipopeptide, surface tension, critical micelle concentration (CMC)