Abstract: To deeply understand the effects of support properties on the performance of Mo-based slurry-phase hydrocracking catalysts, four Mo-based catalysts supported on amorphous silica alumina (ASA), γ-Al2O3, ultra-stable Y (USY) zeolite and SiO2 were prepared by the incipient wetness impregnation method, respectively, and their catalytic performances were compared in the vacuum residue (VR) hydrocracking process. It is found that the Mo/ASA catalyst exhibits the highest VR conversion among the different catalysts, indicating that both the appropriate amount of acid sites, especially B acid sites and larger mesoporous volume of ASA can enhance the VR hydrocracking into light distillates. Furthermore, Mo catalysts supported on the different supports show quite different product distributions in VR hydrocracking. The Mo/ASA catalyst provides higher yields of naphtha and middle distillates and lower yields of gas and coke compared with other catalysts, it is attributed to the highest MoS2 slab dispersion, the highest sulfuration degree of Mo species, and the most Mo atoms located at the edge sites for the Mo/ASA catalyst, as observed by HRTEM and XPS analyses. These features of Mo/ASA are beneficial for the hydrogenation of intermediate products and polycyclic aromatic hydrocarbons to restrict the gas and coke formation.