Abstract: Tight oil resources are abundant in the world. It is very important to strengthen the research on the development theory and technology of tight oil reservoirs for ensuring national energy security. Natural gas huff-n-puff can effectively improve the oil recovery of tight oil reservoirs. However, the pore-scale oil production characteristics and the mechanisms of natural gas huff-n-puff in matrix-fracture cores are poorly understood. The influence degree of important factors on oil recovery is not clear and the interactions between factors are rarely considered. In this paper, the oil production characteristics and mechanisms of natural gas huff-n-puff in tight cores with different fracture lengths were quantitatively analyzed by combining nuclear magnetic resonance (NMR) with numerical simulation technology. The influencing factors and their interactions were evaluated by the response surface method (RSM). The results show that tight cores mainly consist of medium pores (0.1–1 μm) and small pores (0.01–0.1 μm). The fracture mainly increases the proportion of macro-pores (1–10 μm) and medium pores. In the natural gas huff-n-puff process, crude oil from macro-pores (1–10 μm) and medium pores is mainly developed, and the contribution percentage of crude oil in medium pores to oil recovery is the largest, up to 98.28%. The position of gas–oil contact (GOC) moves deeper as the number of huff-n-puff cycles increases. The contents of CH4 and CO2 in the oil phase remain at a high level within the GOC, while between the GOC and the component sweep front, the contents of CH4 and CO2 in the oil phase decrease with the increase in dimensionless distance. The gas component sweep volume is increasing with the increase in fracture length. Moreover, the injected natural gas mainly extracts C3–C10 components from crude oil. The reduction law of crude oil viscosity is consistent with the migration laws of CH4 components along the path. Compared with soaking time and gas diffusion coefficient, the injection pressure is the most significant factor underlying the recovery of natural gas huff-n-puff in tight cores. Besides the influence of single-factor, the interaction effects of gas injection pressure and diffusion also should be considered to determine the huff-n-puff parameters in the field implementation of natural gas huff-n-puff in tight reservoirs after fracturing.