Abstract: Radial borehole fracturing that combines radial boreholes with hydraulic fracturing is anticipated to improve the output of tight oil and gas reservoirs. This paper aims to investigate fracture propagation and pressure characteristics of radial borehole fracturing in multiple layers. A series of laboratory experiments with artificial rock samples (395 mm × 395 mm × 395 mm) was conducted using a true triaxial fracturing device. Three crucial factors corresponding to the vertical distance of adjacent radial borehole layers (vertical distance), the azimuth and diameter of the radial borehole are examined. Experimental results show that radial borehole fracturing in multiple layers generates diverse fracture geometries. Four types of fractures are identified based on the connectivity between hydraulic fractures and radial boreholes. The vertical distance significantly influences fracture propagation perpendicular to the radial borehole axis. An increase in the vertical distance impedes fracture connection across multiple radial borehole layers and reduces the fracture propagation distance along the radial borehole axis. The azimuth also influences fracture propagation along the radial borehole axis. Increasing the azimuth reduces the guiding ability of radial boreholes, which makes the fracture quickly curve to the maximum horizontal stress direction. The breakdown pressure correlates with diverse fracture geometries observed. When the fractures connect multi-layer radial boreholes, increasing the vertical distance decreases the breakdown pressure. Decreasing the azimuth and increasing the diameter also decrease the breakdown pressure. The extrusion force exists between the adjacent fractures generated in radial boreholes in multiple rows, which plays a crucial role in enhancing the guiding ability of radial boreholes and results in higher breakdown pressure. The research provides valuable theoretical insights for the field application of radial borehole fracturing technology in tight oil and gas reservoirs.