Tight sand gas is an important unconventional gas resource occurring widely in different petroleum basins. In coal-bearing formations of the Upper Triassic in the Sichuan Basin and the Carboniferous and Permian in the Ordos Basin, coal measure strata and tight sandstone constitute widely distributed source–reservoir assemblages and form the basic conditions for the formation of large tight sand gas fields. Similar to most tight gas basins in North America, the Sichuan, and Ordos Basins, all experienced overall moderate uplift and denudation in Meso- Cenozoic after earlier deep burial. Coal seam adsorption principles and actual coal sample simulation experiment results show that in the course of strata uplift, pressure drops and desorption occurs in coal measure strata, resulting in the discharge of substantial free gas. This accounts for 28 %–42 % of total gas expulsion from source rocks. At the same time, the free gases formerly stored in the pores of coal measure source rocks were also discharged at a large scale due to volumetric expansion resulting from strata uplift and pressure drop. Based on experimental data, the gas totally discharged in the uplift period of Upper Paleozoic in the Ordos Basin, and Upper Triassic Xujiahe Formation in the Sichuan Basin is calculated as (3–6) 9 108 m3/km2. Geological evidence for gas accumulation in the uplift period is found in the gas reservoir analysis of the above two basins. Firstly, natural gas discharged in the uplift period has a lighter carbon isotope ratio and lower maturity than that formed in the burial period, belonging to that generated at the early stage of source rock maturity, and is absorbed and stored in coal measure strata. Secondly, physical simulation experiment results at high-temperature and high-salinity inclusions, and almost actual geologic conditions confirm that substantial gas charging and accumulation occurred in the uplift period of the coal measure strata of the two basins. Diffusive flow is the main mode for gas accumulation in the uplift period, which probably reached 56 9 1012 m3 in the uplift period of the Xujiahe Formation of the Sichuan Basin, compensating for the diffusive loss of gas in the gas reservoirs, and has an important contribution to the formation of large gas fields. The above insight has promoted the gas resource extent and potential of the coal measure tight sand uplift area; therefore, we need to reassess the areas formerly believed unfavorable where the uplift scale is large, so as to get better resource potential and exploration prospects.