Abstract: Hydrocarbons are one of the important fluids within the Earth’s crust, and different biotic and abitoic processes can generate hydrocarbon during geological periods. Tracing the sources and sinks of hydrocarbons can help us better understand the carbon cycle of the earth. In this study, an improved approach of adsorbed hydrocarbons extraction from sediments was established. The improved thermal desorption approach, compound-specific isotope analysis and position-specific isotope analysis were integrated to investigate the molecular and intramolecular isotope fractionation between trace hydrocarbon gases within sediments and geological hydrocarbon deposits. The isotopic compositions of the terminal position carbon of propane (δ13Cterminal) serves as a correlation indicator between trace hydrocarbon gases within sediments and geological hydrocarbon deposits. The tight sandstone gas from the Turpan-Hami Basin is a first case study for the application of this novel method to trace hydrocarbon origins. The results showed that the hydrocarbons in the tight sandstone gases in the study area most likely originated from humic organic matter (type III kerogen) at an early mature stage. δ13Cterminal values of the thermally desorbed propane gases from different source rocks were distinguishable and the values of the tight sandstone gases significantly overlap with those of the Lower Jurassic Sangonghe source rocks, suggesting their genetic relationship. Overall, the results provided novel position-specific carbon isotopic constraints on origins of hydrocarbons.